Anti-E3 ubiquitin-protein ligase UHRF1

Application

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Detect UHRF1 using this rabbit polyclonal antibody, Anti-E3 ubiquitin-protein ligase UHRF1 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF1 in human breast cancer tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human E3 ubiquitin-protein ligase UHRF1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected E3 ubiquitin-protein ligase UHRF1 in 10 µg of HEK293 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~97 kDa observed