Application

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Western blot: 1:2000 using ECL

Immunohistochemistry: 1:1000

Optimal working dilutions must be determined by the end user.

Detect NMDAR1 Splice Variant C2 using this Anti-NMDAR1 Splice Variant C2 Antibody validated for use in IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Peptide corresponding to the C-terminus of the rat NMDAR1 C2′ splice variant.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 µL of PBS. Contains no preservatives.

Specificity

Specific for the NMDAR1 containing the C2′ splice variant insert. The antibody does not recognize the NMDAR1 subunits of the NMDA receptor that do not contain the C2′ insert. By Western blot the antibody recognizes a protein with molecular weight of 120 kDa. Also labels a single higher molecular weight band that may be a posttranslationally modified form of the protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.