ANTI-CHOLINE ACETYLTRANSFERASE ANTIBODY

Application

Detect Choline Acetyltransferase using this Anti-Choline Acetyltransferase Antibody validated for use in ELISA, IH.

Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.

Optimal working dilutions must be determined by the end user.

Immunogen

A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, mouse primate and human ChAT.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Lyophilized. Contains no preservative. Reconstitute with 20 µL of sterile distilled water. Centrifure to remove any insoluble material.

Specificity

Choline acetyltranferase. The antibody will stain cholinergic neurons in the rat central and peripheral nervous systems.

Storage and Stability

Maintain lyophilized material dry at -70°C for up to 12 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.