IRON REGULATORY PROTEIN 2; RBX-50UG

Application

Detect Iron Regulatory Protein 2 using this Anti-Iron Regulatory Protein 2 Antibody validated for use in ELISA & WB.

General description

Iron regulatory proteins (IRP-1 and IRP-2) are cytoplasmic mRNA-binding proteins that control intracellular iron levels by regulating the translation of ferritin, Tfrs, and other proteins congaing iron-responsive element (IRE) located at the 5′-UTR. IRP binds to IREs with high affinity in situation of iron starvation. Binding of IRP to IRE of ferritin represses its transcription. However, when cells are iron replete, IRPs lose their capacity to bind IREs, allowing efficient translation of ferritin and reducing Tfr mRNA half-life. Although IRP1 and IRP-2 share significant protein sequence homology, they differ in tissue distribution and mode of regulation. IRP-2/IRE-BP2 (rat/human 963 aa) is relatively less abundant than IRP-1 and lacks aconitase activity. It has a unique 73-aa insertion in the N-terminus. In the presence of high iron levels, IRP-2 is rapidly targeted to proteasome-mediated degradation. Although, both IRPs bind the consensus IREs, it is also shown that IRP-2 can recognize an exclusive IRE subset.

Immunogen

Synthetic peptide near the N-terminus of human/rat/mouse IRP-2.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

Liquid in PBS with 0.1% BSA and 0.05% sodium azide.

Specificity

Iron regulatory protein 2 (IRP-2). The immunogen sequence has no significant homology with IRP-1.

The immunogen sequence is 100% conserved in human, rat, and mouse IRP-2.