2-METHYLNICOTINIC ACID IMIDAZOLIDE

Application

2-Methylnicotinic acid imidazolide (NAI or mNAI) is an RNA SHAPE probe for live-cell RNA structure profiling. SHAPE -- or selective 2′-hydroxyl acylation analyzed by primer extension -- uses small, electrophilic chemical probes to react with the 2′-hydroxyl group and provides insight to RNA structure and its dynamics in varied cells states or in cells with gene knockouts. It has also been extended to the SHALiPE approach, selective 2′-hydroxyl acylation with lithium ion-based primer extension.

Other Notes

Conditional control of RNA-guided nucleic acid cleavage and gene editingGuidelines for SHAPE Reagent Choice and Detection Strategy for RNA Structure Probing StudiesSnapShot: RNA Structure Probing TechnologiesStructural Analysis using SHALiPE to Reveal RNA G-Quadruplex Formation in Human Precursor MicroRNADetermination of in vivo RNA structure in low-abundance transcripts