Application

pRSFDuet-1 DNA - Novagen has been used as a vector:to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expressionto clone the coding region of Mad1597–719to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs

General description

pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

10 µg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)