Features and Benefits
Highly efficient DNA transfer for both stable and transient transfections Compatibility with both serum-containing and serum-free media Simple protocolno need for media changes Ideal for high-throughput transfection in a multi-well plate format Ideal for retrovirus production for T-cell transduction Works for a multitude of cell types with minimal optimization Provides higher transfection efficiency and lower cytotoxicity than reagents from other suppliers The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates
General description
""I use GeneJuice because it′s ease-of-use, low toxicity and wide range of target cells""Stuart Rulten. Research Fellow. University of Sussex""Since we have been working with GeneJuice, we have saved a lot of time and money!""Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany""I find that Genejuice works for a multitude of cell types with minimal optimization""Caitriona Marie Lyons. PhD Student. University College Cork, IrelandTransfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.
r>Cell lines transfected with GeneJuice® Transfection Reagent
Cell Lines: | Primary Cells: |
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10T1/2r>293Tr>3T3 NIHr>3T3 Swissr>3T3-L1r>A204r>A431r>A549r>alpha TC1-6r>AR 42Jr>As4.1r>AtT-20r>B50r>BC-1r>BC-2r>BC3r>BCBLr>BHK-21r>C3H/10T1/2r>C6r>C2C12 | Caco-2r>Caki-1r>Calpan-1r>Calu-1r>Calu-6r>CCL-131r>CFPAC-1r>Chang Liverr>CHOr>CHO-7r>CHO-IRr>CHO-K1r>COS-1r>COS-7r>CS-1r>CV-1r>Daudir>DDTI MF-2r>DT40r>ECV304r>EL4 | ES-E14TG2ar>EVSCC17Mr>H9c2r>HCT-116r>HEK293r>HeLar>HeLa Br>HeLa T4r>Hep 3B2.1-7r>HepG2r>Hepa 1-6r>Ht-29r>HTB-37r>HTB-45r>Huh-7r>HUVECr>IC21r>IEC-6r>JEG-3r>Jurkatr>KB | L57-3-11r>L-6r>L-929r>MA-10r>McA-RH7777r>MCF-7r>MCF-10-2Ar>MDCKr>Melanocyter>MG-63r>Neuro 2Ar>Neuroblastomar>NRKr>NT2/D1r>OV-1063r>OVCAR3r>P4r>P19r>PC12r>PA317r>PAM212 | PS-1r>R2Cr>RAW 264.7r>RBL-2H3r>RMP-41r>SAOS-2r>SC-1r>Schneider line2r>SK-N-MCr>SK-N-SHr>SKOV3r>STOr>SW-480r>SW-837r>T3M4r>TM4r>U937r>UCDr>Veror> | Aortic smooth muscle cellsr> Astrocytesr> Angioblastsr> Chondrocytesr> Chromaffin cellsr> Epithelial cells:r> mammaryr> prostater> trachealr> Fibroblastsr> Keratinocytesr> |
Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
GeneJuice® is a registered trademark of EMD Chemicals Inc.
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Packaging
0.3 ml in Plastic ampoule
1, 10, 5 ml in Glass bottle
Preparation Note
For protocol, please click (here)
Warning
Toxicity: Flammable (J)
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