Application

Oxidase Test is an important differential procedure which should be performed on all gram-negative bacteria that are to be identified.

Packaging

Discs contain N,N-dimethyl-p-phenylenediamine oxalate and A-naphthol.

Principle

Oxidase Test is based on the principle that certain bacteria produce indophenol blue from the oxidation of dimethyl-p-phenylenediamine and α-naphthol. In presence of the enzyme cytochrome oxidase (gram-negative bacteria), the N,N-dimethyl-p-phenylenediamine oxalate and α-naphthol react to indophenol blue.