Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetA potent mitogen

General description

SPC is the deacylated derivative of sphingomyelin known to accumulate in neuropathic Niemann-Pick disease type A. A potent mitogen that increases intracellular free Ca²⁺ and free arachidonate through pathways that are only partially PKC-dependent. Stimulates DNA-binding activity of the transcription activator protein AP-1. Rapidly induces tyrosine phosphorylation of focal adhesion kinase (p125^FAK) and paxillin and causes a rapid and transient activation of MAP kinase in Swiss/3T3 cells. Also induces a unique reorganization of the actin cytoskeleton.

SPC is the deacylated derivative of sphingomyelin known to accumulate in neuropathic Niemann-Pick disease type A. A potent mitogen that increases intracellular free Ca²⁺ and free arachidonate through pathways that are only partially PKC-dependent. Stimulates DNA-binding activity of the transcription activator protein AP-1. Rapidly induces tyrosine phosphorylation of focal adhesion kinase (p125^FAK) and paxillin and causes a rapid and transient activation of MAP kinase in Swiss 3T3 cells. Also induces a unique reorganization of the actin cytoskeleton.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Berger, A., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 5885.Dettbarn, C., et al. 1995. Brain Res.669, 79.Seufferlein, T. and Rozengurt, E. 1995. J. Biol. Chem. 270, 24343.Seufferlein, T. and Rozengurt, E. 1995. J. Biol. Chem. 270, 24334.

Packaging

10 mg in Plastic ampoule

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Warning

Toxicity: Standard Handling (A)