Biochem/physiol Actions

Primary TargetSerine and cysteine proteases, but not metalloproteases

Cell permeable: no

Reversible: no

Product does not compete with ATP.

General description

A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases. Recommended for use with mammalian cell lysates and tissue extracts. Provided as a single vial or a set of 10 vials. When reconstituted each vial contains a 100X stock solutions. When diluted to 1X the cocktail contains the following components:

A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases. Reconstitute each vial with 1 ml H₂O to obtain a 100X stock solution. 1X stock solution contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616370), 1 µM E-64 (Cat. No. 324890), and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Supplied with a data sheet.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

10 vl in Glass bottle

1 ml in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Reconstitute each vial with 1 ml H₂O to obtain a 100X stock solution.

Specificity

Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases.

Warning

Toxicity: Standard Handling (A)