Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetMembrane impermeable DNA intercalator

General description

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Belloc, F., et al. 1994. Cytometry17, 59.Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.Pollice, A.A., et al. 1992. Cytometry13, 432.

Packaging

5 ml in Plastic ampoule

Physical form

50 µg/ml in PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)