Biochem/physiol Actions

Cell permeable: yes

General description

A cell-permeable isoquinoline derivative that acts as a specific AMP competitive inhibitor of AMP deaminase (IC₅₀ = 38, 27, and 24 nM for human, rat, and mice, respectively). Potentiates low frequency electrical stimulation induced increase in AMP level, AMP:ATP ratio, and phosphorylation of AMPK (at Thr172), and acetyl-CoA carboxylase (at Ser218) in contracting rat epitrochlearis muscle. However, it does not affect basal AMPK activity and contraction-stimulated glucose uptake. Preincubation with this compound reduces basal IMP levels, but does affect the contraction-induced increases in IMP, adenosine, and inosine in any appreciable manner.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Admyre, T., et al. 2014. Chem. Biol.21, 1486.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)