Peroxiredoxin Inhibitor; Conoidin A

Biochem/physiol Actions

Reversible: no

Target IC₅₀: 23 µM for blocking TgPrxII hyperperoxidation activity

Primary TargetPeroxiredoxin

Cell permeable: yes

General description

A cell-permeable dibromomethylquinoxaline-dioxide compound with nematicidal properties that covalently binds to the peroxidatic cysteine of peroxiredoxin II in Toxoplasma gondii (TgPrxII) and irreversibly blocks its hyperperoxidation activity (IC₅₀ = 23 µM). Also shown to alkylate or crosslink catalytic cysteines of wild type AcePrx-1 in Ancylostoma ceylanicum and human PrxII and PrxIV to inhibit their catalytic activity with similar efficiency. Ineffective towards mitochondrial hPrxIII.Please note that the molecular weight for this compound is batch-specific due to variable water content.

A cell-permeable dibromomethylquinoxaline-dioxide compound with nematicidal properties that covalently binds to the peroxidatic cysteine of peroxiredoxin II in Toxoplasma gondii (TgPrxII) and irreversibly blocks its hyperperoxidation activity (IC₅₀ = 23 µM). Also shown to alkylate or crosslink catalytic cysteines of wild type AcePrx-1 in Ancylostoma ceylanicum and human PrxII and PrxIV to inhibit their catalytic activity with similar efficiency. Ineffective towards mitochondrial hPrxIII.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Nguyen, J.B., et al. 2013. Chem. Biol.20, 991.Haraldsen, J. D., et al. 2009. Org. Biomol. Chem.7, 3040.Carey, K.L., et al. 2004. Proc. Natl. Acad. Sci. USA101, 7433.

Packaging

25 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 2 weeks at -70°C.

Use only fresh DMSO for reconstitution.

Warning

Toxicity: Standard Handling (A)