Urea, BioUltra, for molecular biology, >=99.5% (T)

Code: 51456-500G D2-231

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already ...


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€93.50 500G
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Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

λ8 M in H2O
anion tracessulfate (SO42-): ≤10 mg/kg, chloride (Cl-): ≤5 mg/kg
assay99% (T)
cation tracesBi: ≤5 mg/kg, Na: ≤50 mg/kg, Mo: ≤5 mg/kg, Al: ≤5 mg/kg, K: ≤50 mg/kg, As: ≤0.1 mg/kg, Li: ≤5 mg/kg, Cr: ≤5 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Mg: ≤5 mg/kg, Ca: ≤10 mg/kg, Pb: ≤5 mg/kg, Cu: ≤5 mg/kg, Ba: ≤5 mg/kg, Fe: ≤5 mg/kg, Zn: ≤5 mg/kg, Ni: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg
density1.335 g/mL at 25 °C (lit.)
formpowder or crystals
Gene Informationhuman ... CA1(759), CA2(760)rat ... Ppm1a(24666)
gradefor molecular biology
ign. residue≤0.01% (as SO4)
impuritiesphosphatases, none detected, RNases, none detected, ﹤0.005% formaldehyde (HCHO), proteases, none detected, insoluble matter, passes filter test, ≤0.1% biuret, DNases, none detected
InChI keyXSQUKJJJFZCRTK-UHFFFAOYSA-N
InChI1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
loss≤0.1% loss on drying, 20 °C (HV)
mp132-135 °C (lit.)
pH8.0-10.0 (20 °C, 8 M in H2O)
product lineBioUltra
Quality Level200
SMILES stringNC(N)=O
solubilityH2O: 8 M at 20 °C, clear, colorless
UV absorptionλ: 280 nm Amax: ≤0.02, λ: 260 nm Amax: ≤0.03
Cas Number57-13-6
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