Name: Glycerol, BioUltra, for molecular biology, anhydrous, >=99.5% (GC)
Brand: SIGMA-ALDRICH
SKU: 49767-100ML
Price: 69.62 EUR

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’;s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Biochem/physiol Actions

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Glycerol has been used as a supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium. a fuel during designing enzymatic biofuel cell. a liquid composite matrix with 4-HCCA and 3-aminoquinoline for analysis of neutral and acidic glycans. a matrix for fast atom bombardment MS. may be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Other Notes

Component of loading buffer in agarose gel electrophoresis of nucleic acids previously denatured with glyoxal; Preparation of phage and plasmid DNA, for the storage of pure cultures

Packaging

1 L in glass bottle

100, 250 mL in glass bottle

λ	5 M in H₂O
anion traces	sulfate (SO₄^2-): ≤10 mg/kg, chloride (Cl^-): ≤1 mg/kg
assay	≥99.5% (GC)
autoignition temp.	698 °F
bp	182 °C/20 mmHg (lit.)
cation traces	Cd: ≤1 mg/kg, Fe: ≤1 mg/kg, Ag: ≤5 mg/kg, Mg: ≤1 mg/kg, Bi: ≤1 mg/kg, As: ≤0.1 mg/kg, Mo: ≤1 mg/kg, Al: ≤1 mg/kg, Ni: ≤1 mg/kg, Cr: ≤1 mg/kg, Ca: ≤5 mg/kg, Na: ≤20 mg/kg, Tl: ≤5 mg/kg, K: ≤20 mg/kg, Zn: ≤1 mg/kg, Pb: ≤1 mg/kg, Co: ≤1 mg/kg, Ba: ≤1 mg/kg, NH₄⁺: ≤5 mg/kg, Cu: ≤1 mg/kg, Mn: ≤1 mg/kg, Sr: ≤1 mg/kg, Li: ≤1 mg/kg
density	1.25 g/mL (lit.)
form	viscous liquid
grade	for molecular biology
ign. residue	≤0.1%
impurities	phosphatases, none detected, RNases, none detected, proteases, none detected, insoluble matter, passes filter test, DNases, none detected
InChI key	PEDCQBHIVMGVHV-UHFFFAOYSA-N
InChI	1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
mp	20 °C (lit.)
pH	5.5-8 (25 °C, 5 M in H₂O)
product line	BioUltra
Quality Level	200
refractive index	n20/D 1.474, n20/D 1.474 (lit.)
SMILES string	OCC(O)CO
solubility	H₂O: 5 M at 20 °C, clear, colorless
UV absorption	λ: 280 nm A_max: 0.04, λ: 260 nm A_max: 0.05
vapor density	3.1 (vs air)
vapor pressure	﹤1 mmHg ( 20 °C)

Safety Data Sheet for 49767-100ML

Cas Number

56-81-5

This product has met the following criteria to qualify for the following awards:

Glycerol, BioUltra, for molecular biology, anhydrous, >=99.5% (GC)

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