Application
Abberior™ STAR RED labelled cholesterol analogs were used for STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) based measurements of membrane diffusion dynamics. Anti-mouse secondary antibody coupled to Abberior™ STAR RED has been used for STED microscopy imaging in Vero cell line.
General description
Abberior STAR RED is an already well known dye named also KK114 in literature (a scientific web search shows more than 60 publications, a selection is listed below). Please note the minimal background signal due to negligible unspecific binding. A key feature of the dye is its comparably long lifetime of ~3.5ns. The dye works exceptionally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope.Abberior STAR RED can substitute Atto™ 647N, AlexaFluor 647, or Cy®5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference [2] for detailed characteristics.Best results are obtained with freshly prepared samples.
Legal Information
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
6538 is a trademark of American Type Culture Collection
Atto is a trademark of Atto-Tec GmbH
Other Notes
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Suitability
Designed and tested for fluorescent super-resolution microscopy
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