Biochem/physiol Actions

Reversible: yes

Product competes with ATP.

Primary TargetJNK 1, JNK 2, JNK 3

Target IC₅₀: 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3

Cell permeable: yes

General description

A potent, cell-permeable, selective, and reversible inhibitor of c-Jun N-terminal kinase (JNK) (IC₅₀ = 40 nM for JNK-1 and JNK-2 and 90 nM for JNK-3). The inhibition is competitive with respect to ATP. Exhibits over 300-fold greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks the expression of IL-2, IFN-γ, TNF-α, and COX-2 in cells. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun transcription.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bennett, B.L., et al. 2001. Proc. Natl. Acad. Sci. USA98, 13681.Han, Z., et al. 2001. J. Clin. Invest.108, 73.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Physical form

A 50 mM (5 mg/454 µl) solution of JNK Inhibitor II (Cat. No. 420119) in DMSO.

Reconstitution

Following initial use, aliquot and refrigerate (4°C) for short-term storage or freeze (-20°C) for long-term storage.

Warning

Toxicity: Irritant (B)