Application

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

Biochem/physiol Actions

Potency: ≥1000 U/mg

General description

Sterile liquid formulation of Cat. No. 400050.

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sugimoto, K., et al. 1994. Plant J.5, 863.Lee, M.G., and van der Ploeg, L.H. 1991. Gene105, 255.Salauze, D., et al. 1990. Antimicrob. Agents Chemother.34, 1915.Cullen, D., et al. 1987. Gene57, 21.Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.Santerre, R.F., et al. 1984. Gene30, 147.Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Packaging

20, 5, 50 ml in Alu drum

Physical form

50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)