General description

Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like pro-hormone convertase (PC) family. Members of this family cleave pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins. The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus.

This preparation was subjected to UV irradiation for 5 min to inactivate any residual vaccinia virus.

Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Supplied as a crude preparation of soluble furin obtained from cytoplasmic extracts. Furin is a type I membrane protein that processes stromelysin-3 (MMP-11) and MT1-MMP. It cleaves pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224.Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326.Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211.Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953.Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.

Packaging

50 µL in Plastic ampoule

Physical form

In 20 mM Bis-Tris buffer, 1 mg/ml BSA, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of furin that will cleave 1.0 pmol AMC from the substrate pGlu-Arg-Thr-Lys-Arg-AMC per min at 30°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)