Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

General description

Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.Schwartz, Y., et al. 1992. Endocrinology131, 772.Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.Tsien, R.Y. 1980. Biochemistry19, 2396.

Packaging

Packaged under inert gas

1 ml in Plastic ampoule

Physical form

A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.

Warning

Toxicity: Irritant (B)