Dihydroethidium

Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Primary TargetUseful fluorogenic probe for the detection of reactive oxygen species (ROS)

Cell permeable: yes

General description

A cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell's DNA, staining the nucleus with a bright red fluorescence.

A cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Haugen, T.S., et al. 1999. Eur. Respir. J.14, 1100.Mancini, M., et al. 1997. J. Cell Biol.138, 449.Carter, W.O., et al. 1994. J. Leukoc. Biol.55, 253.Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.Perticarari, S., et al. 1991. Cytometry 12, 687.Bucana, C.D., et al. 1990. Exp. Cell Res.190, 69.Rothe, G., and Valet, G. 1990. J. Leukoc. Biol.47, 440.

Packaging

25 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Irritant (B)