General description

Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.

Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481.Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.

Packaging

25 mu in Plastic ampoule

10 mu in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Unit Definition

One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.

Warning

Toxicity: Standard Handling (A)