Biochem/physiol Actions
Reversible: no
Target IC50: 50-200 µM against Bax mediated apoptosis
Primary TargetBax mediated apoptosis
Product does not compete with ATP.
Cell permeable: yes
General description
A cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).
A cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun.321, 961.Sawada, M., et al. 2003. Nature Cell Biol.5, 352.
Packaging
Packaged under inert gas
5 mg in Plastic ampoule
Reconstitution
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.
Sequence
H-Val-Pro-Met-Leu-Lys-OH
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: