Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetNitroxyl anion (NO-) donor

General description

A nitroxyl anion (NO^-) donor that spontaneously, in the presence of oxygen and Cu²⁺ and Fe³⁺, dissociates in a pH-dependent, first-order process to liberate 0.54 moles of NO per mole of the parent compound. Half-life (t_½) of NO release = 2.3 min. (in 100 mM phosphate buffer, pH 7.4 at 37°C). Induces relaxation of norepinephrine-constricted rabbit aorta (EC₅₀ = 590 nM). Angeli′s Salt produces nitroxyl anion (NO^-) that can be converted to NO in the presence of heme.

A nitroxyl anion (NO^–) donor that spontaneously dissociates in a pH dependent first order process to liberate 0.54 moles of NO per mole of the parent compound. Half-life (t_½) in 100 mM phosphate buffer, pH 7.4 is 2.3 minutes at 37°C. Decomposition is nearly instantaneous at pH 5.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Miranda, K.M., et al. 2001. J. Biol. Chem.276, 1720.Hrinczenko, B.W., et al. 2000. Br. J. Haematol.110, 412.Nelli, S., et al. 2000. Br. J. Pharmacol.131, 356.Ma, X.L., et al. 1999. Proc. Natl. Acad. Sci. USA96, 14617.Keefer, L.K., et al. 1996. Methods Enzymol.268, 281.Fukuto, J.M., et al. 1993. Biochem. Biophys. Res. Commun.196, 707.Maragos, C.M., et al. 1991. J. Med. Chem.34, 3242.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution in alkaline solution, store at -20°C. This product is stable for 6 months as supplied. Alkaline stock solutions are stable for up to 24 h at -20°C. Unstable in acidic or neutral pH buffers.

Warning

Toxicity: Standard Handling (A)