OXFORD-LISTERIA-SELECTIVE-AGAR (BASE) FOR MICROBIOLOGY

Analysis Note

Base tested with Oxford-Listeria-selektiv-supplement art. no. 1.07006.Appearance (clearness): clearAppearance (color): blueish-brown to greenish-brownpH-value (25 °C): 6.8 - 7.2Growth promotion test in accordance with the current version of DIN EN ISO 11133.Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)): Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)): Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)): Colony count (Listeria innocua ATCC 33090 (WDCM 00017)): Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 50Growth Escherichia coliATCC 25922 (WDCM 00013): total inhibitionGrowth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibitionGrowth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibitionGrowth (Candida albicans ATCC 10231 (WDCM 00054)): partial inhibitionBlack zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zoneBlack zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zoneBlack zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zoneIncubation: up to 48 hours at 35 ± 1 °C, aerobicA recovery rate of 50 % is equivalent to a productivity value of 0.5.The indicated colony counts result from the sum of a triple determination.Reference media: Tryptic Soy Agar

Application

GranuCult^® Oxford Agar (Base) is a selective agar for the isolation and detection of Listeria monocytogenes in milk and milk products and other food products.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

GranuCult^® Oxford Agar (Base) formulation is based on Columbia Agar with the addition of lithium chloride, acriflavin, colistin sulfate, cefotetan, cycloheximide, and fosfomycin. These ingredients suppress the growth of the common bacteria (e.g., Gram-negative bacteria and a greater part of Gram-positive bacteria). Lithium chloride is one of the ingredients of Oxford Agar base, while the other substances are derived from the Oxford Listeria Selective Supplement (1.07006). Listeria monocytogenes hydrolyses esculin to esculetin and forms a black complex with iron (III) ions, thereby producing brown-green colored colonies with a black halo.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500 g in Plastic bottle