Analysis Note

Appearance (clearness): turbid with sedimentAppearance (colour): greenpH-value (25 °C): 7.8 - 8.2Growth promotion test in accordance with the current version of DIN EN ISO 11133.Mixed inoculum of Salmonella, E.coli and P.aeruginosaAdditionaly monoculture of E.coli and E.faecalisConfirmation on XLD agar resp. Tryptic soy agarInoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 coloniesGrowth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 coloniesGrowth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): ﹤100 coloniesGrowth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): ﹤100 coloniesGrowth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ﹤10 coloniesGrowth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ﹤10 coloniesIncubation:MKTTn broth: 24 ± 3 hours at 37 ± 1 °C aerobicConfirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic

Application

MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth (base) is used for the selective enrichment of Salmonella from food, animal feed, and other materials.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

MKTTn (MULLER-KAUFFMANN Tetrathionate Novobiocin) broth is a selective medium suitable for the enrichment and isolation of Salmonella. Tetrathionate in the broth suppresses the growth of coliform and other enteric bacteria. It is produced from thiosulfate by adding iodine to the culture medium. Salmonella, Proteus, and some other species of bacteria can reduce tetrathionate. Calcium carbonate buffers the sulphuric acid, which is generated when tetrathionate is reduced. Bile promotes the growth of Salmonella, but largely inhibits the accompanying bacteria. Brilliant green and novobiocin suppress the growth of primarily Gram-positive bacteria.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

500 g in Plastic bottle