Analysis Note

Cell nuclei: dark blue to dark violetCytoplasma, intercellular substances: pink to redErythrocytes: yellow to orange

Application

For microscopy

General description

M-FREEZE^™ Cryoembedding media - for microscopy, is used for the routine preparation of specimens of unfixed tissue (native material) of human origin for preparing cryosections, e. g. in the frozen-section diagnosis procedure, for immuno-histology, and for enzyme diagnostic methods. The PEG- and PVA-based aqueous formulation helps to achieve precise and stable positioning of tissue specimens on the holder. This support matrix can be used to quickly freeze and support tissue for sectioning at 3 µm and up, with no cracking of the matrix at a variety of temperatures [-150 °C using nitrogen-cooled isopentane, CO2, or gradual freezing in the cryostat (-35 to -15 °C)]. The 100 ml bottle provides approx. 250 applications. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Legal Information

M-FREEZE is a trademark of Merck KGaA, Darmstadt, Germany