Application

Research Sub CategoryInflammatory DiseaseImmune ResponseAutoimmune disease

This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-13 v2 kit, #03-0109-02:
Improved options for sample prep and incubation
Lyophilization of the standard provides the kit at one storage condition
Overnight incubation can be used
Increased reagent volumes to accommodate automation
Validated to run on both SMCxPRO^® and Erenna™

Research CategoryInflammation & Immunology

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC™ Human IL-13 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-13 in human serum and plasma.

Components

Assay BufferHuman IL-13 BeadsStandard DiluentHuman IL-13 Detection AntibodyHuman IL-13 Standard10X Wash BufferBuffer DElution Buffer B

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The SMC^® Human IL-13 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna™ and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.

Interleukin 13 (IL-13) is secreted by a number of cells including T helper cells, CD4 cells and a various white blood cells and is thought to be a mediator of allergic inflammation in many tissues via the induction of IgE synthesis by B cells. Although primarily associated with the induction of airway disease, IL-13 has also been shown to inhibit the production of proinflammatory cytokines by monocytes and macrophages suggesting it has important anti-inflammatory properties.

Legal Information

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

Specifications

Minimum of 5 serum samples spiked with average recovery of 90% with a range of 86-102% and 5 plasma samples spiked with average recovery of 92% with a range of 87-96%.

Specificity

Although IL-13 was detectable in non-human primate, rat, canine, and guinea pig samples, the assay has not been validated for these species.