Application

Research CategoryNeuroscienceMetabolism

Research Sub CategoryInflammation & Autoimmune MechanismsMetabolismObesity

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC™ Amyloid Beta 1-40 (Aβ40) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Aβ40 in human, mouse and rat cerebrospinal fluid (CSF) and plasma.

Components

Assay BufferSMC™ Amyloid Beta 1-40 Coated BeadsSMC™ Amyloid Beta Standard DiluentSMC™ Amyloid Beta Detection AntibodySMC™ Amyloid Beta 1-40 Standard10X Wash BufferBuffer DElution Buffer B

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Amyloid Beta refers to the short peptides that are released into circulation following the proteolysis of the transmembrane protein amyloid precursor protein (APP), whose function is unclear but thought to be involved in neuronal development and function. The most common peptides generated from APP cleavage are Aβ1-40 and Aβ1-42, with the latter isoform implicated in the formation of toxic oligomers, fibrils, and plaques in the diseased brain. Reduced levels of soluble Aβ1-42 in the cerebrospinal fluid (CSF) are associated with Alzheimer's disease (AD), leading to the adoption of this peptide as an AD biomarker. The presence of AB40, AB42 in patient CSF and plasma changes as an individual ages, and increases in AB42 levels, and more importantly, the AB42/AB40 ratio, are recognized as correlating with dementia and the onset of Alzheimer's.

The SMC^® Amyloid Beta 1-40 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna™ and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.

Legal Information

SMC is a registered trademark of Singulex

Specifications

Minimum of 5 CSF samples spiked with average recovery of 102% with a range of 95-106% and 5 plasma samples spiked with average recovery of 97% with a range of 92-100%.