Vacuolin-1

Biochem/physiol Actions

Reversible: no

Primary TargetCa2+-dependent fusion of lysosomes with the plasma membrane

Product does not compete with ATP.

Cell permeable: yes

General description

A cell-permeable triazine based compound that inhibits Ca²⁺-dependent fusion of lysosomes with the plasma membrane and the release of lysosomal content, without affecting enlargeosomes or other membrane-bound organelles. It does not disturb the organization of the actin or tubulin cytoskeletal network, nor does it interfere with membrane traffic along the constitutive pathway from the ER to the plasma membrane.

A cell-permeable triazine-based compound that inhibits Ca²⁺-dependent exocytosis of lysosomes induced by ionomycin or plasma membrane wounding. Does not affect the resealing process and the exocytosis of enlargeosomes. It also does not disturb the organization of the actin or tubulin cytoskeletal network or interfere with membrane traffic along the constitutive pathway from the ER to the plasma membrane.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cerny, J., et al. 2004. EMBO Rep.5, 883.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)