Cell Line Description

DerivationPrimary Cell Type: Fibroblast of dermisDevelopmental Stage: AdultPassage Number: P4Reprogramming Method: Sendai Virus (KLF4, MYC, POU5F1, SOX2)Other: From the same donor as UNEWi022-B, UNEWi022-CCharacterizationMarker Expression: NANOG(+), TRA160(+), SSEA4(+), SSEA1(-)Differentiation Potential: Ectoderm (TUBB3+), Endoderm (AFP+), Mesoderm (ACTA2+)Microbiology / Virus ScreeningHIV1: NegativeHIV2: NegativeHepatitis B: NegativeHepatitis C: NegativeMycoplasma: NegativeMicrobial Growth: None DetectedViability: Viable Post-CryopreservationGenotypingKaryotype: P14, Chromosome 12 gain by molecular karyotype SNP arrayGenotype: 16 allele STR profile available upon request

Cell Line Origin

DepositorUniversity of Newcastle

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer’;s Disease, Parkinson’;s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’;s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Legal Information

mTeSR is a registered trademark of WiCell Research Institute, Inc.

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Linkage

Note: EAUA and CLIP must be completed before order fulfillment

Additional Cell Line Info EBiSC Access Use Agreement (EAUA) Cell Line Information Pack (CLIP)

Subculture Routine

Medium: mTeSR^®Passage method: EDTAMatrix: Matrigel^®/Geltrex^®CO₂ concentration: 5%O₂ concentration: 20%Temperature: 37°C