TIGARHUMAN

Code: SRP6244-10UG D2-231

General description

TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2...


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€286.70 10UG
Discontinued
€352.64 inc. VAT

General description

TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Recombinant human TIGAR expressed in E. coli is a 29.9 kDa protein containing 269 amino-acid residues.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Tris, pH 8.0, 200 mM NaCl and 1 mM DTT.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

assay≥95% (SDS-PAGE)
biological sourcehuman
formlyophilized powder
Gene Informationhuman ... TIGAR(57103)
impurities﹤1 EU/µg endotoxin (LAL test)
mol wt29.9 kDa
packagingpkg of 50 µg, pkg of 10 µg
recombinantexpressed in E. coli
shipped inwet ice
storage temp.−20°C
UniProt accession no.Q9NQ88
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