SPAM1HUMAN

Code: SRP6272-10UG D2-231

General description

Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SP...


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€597.97 10UG
Discontinued
€735.50 inc. VAT

General description

Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1→4)-linkages between N – acetyl – β – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Physical form

Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

assay≥92% (SDS-PAGE)
biological sourcehuman
conjugate(Glycosaminoglycan)
formlyophilized powder
Gene Informationhuman ... SPAM1(6677)
impurities﹤1 EU/µg endotoxin (LAL test)
mol wtobserved mol wt 64-66 kDa (DTT-reduced. Protein migrates due to different glycosylation. Leu 36 is the predicted N-terminus.), calculated mol wt 52.1 kDa
packagingpkg of 50 µg, pkg of 10 µg
recombinantexpressed in HEK 293 cells
shipped inwet ice
storage temp.−20°C
tag6-His tagged (C-terminus)
UniProt accession no.P38567
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