General description

Secreted frizzled-related protein 1 (SFRP1) also known as Secreted apoptosis-related protein 2 (SARP2), FRP, FRP1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1, SFRP2, SFRP3, SFRP4, SFRP5) that act as extracellular signaling ligands. SFRP1 / SARP-2 is widely expressed and absent from lung, liver and peripheral blood leukocytes, and with the highest levels in heart and fetal kidney. SARP2 / SFRP-1 is a 35 kDa prototypical member of the SFRP family. SARP2 / FRP-1 acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations.

Physical form

Lyophilized from 0.22 µm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.