Biochem/physiol Actions

DLL4 (delta like canonical Notch ligand 4) functions to specifically activate the Notch-1 and Notch-4 receptors. The Notch signaling pathway regulates endothelial-cell differentiation, proliferation and apoptosis, and is essential for the development, maintenance and remodeling of the vascular system. Targeted deletion of the DLL4 gene in mice resulted in severe vascular defects and death before birth. Up-regulation of DLL4 expression has been implicated in the vascular development of certain tumors. Recombinant human sDLL4 is a 54 160;kDa glycoprotein containing 498 amino-acid residues.

General description

Human sDLL4 comprises the extracellular signaling domain of DLL, a member of a structurally-related family of single-pass type I trans-membrane proteins that serve as ligands for Notch receptors.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 1X PBS, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.