Biochem/physiol Actions

Primary TargetCa2+ uptake into mitochondria in vitro

Target IC₅₀: 184 pM blocking Ca2+ uptake into mitochondria in vitro; and in situ in intact myocytes; Kd = 340 pM for binding mitochondria

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

General description

A cell-permeable oxygen bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 0.34 nM) and specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (for complete block after incubation with ~10 µM of Ru360 for 30 min. Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction even at micromolar levels.

A cell-permeable oxygen-bridged dinuclear ruthenium amine complex that has been shown to bind to mitochondria with high affinity (K_d = 340 pM). Specifically blocks Ca²⁺ uptake into mitochondria in vitro (IC₅₀ = 184 pM) and in situ in intact myocytes (complete block after incubation with ~10 µM of Ru360 for 30 min). Does not affect other cellular Ca²⁺ transport processes involved in cardiac muscle contraction, even at micromolar levels.

Note: 1 set = 10 x 100 µg.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sanchez, J.A., et al. 2001. J. Physiol. 536, 387.Bassani, R.A., et al. 1998. Cell Calcium23, 433.Matlib, M.A., et al. 1998. J. Biol. Chem.273, 10223.Zhou, Z., et al. 1998. J. Physiol.507 (pt 2), 379.Emerson, J., et al. 1993. J. Am. Chem. Soc.115, 11799.Ying, W.-L., et al. 1991. Biochemistry30, 4949.

Packaging

1 set in Glass bottle

Packaged under inert gas

500 µg in Plastic ampoule

Reconstitution

Unstable in solution; reconstitute just prior to use.

Warning

Toxicity: Standard Handling (A)