Application

For research use only. Not for use in diagnostic procedures.The Cellular DNA Fragmentation ELISA is for the quantification of BrdU-labeled DNA fragments either released from cells during necrosis or cell-mediated cytotoxicity, or within the cytoplasm of apoptotic cells.

Features and Benefits

Nonradioactive assay system Sensitive (1 x 10³ cells/well) Low assay background No additional dilution steps required No washing of the cells required Results obtained correlate to those obtained by standard methods Denaturation/fixation of DNA in the microplate by microwave irradiation Fast (4.5 - 5.5 hours)

General description

The Cellular DNA Fragmentation ELISA is a photometric enzyme-linked immunosorbent assay (ELISA) for the detection of 5’;-bromo-2’;-deoxy-uridine (BrdU)-labeled DNA fragments in culture supernatants and cell lysates. BrdU is used as a metabolic labeling agent by the nuclear DNA of target cells. This BrdU-labeled DNA can be detected easily and quantified using a monoclonal antibody against BrdU in an ELISA. This kit is a non-radioactive alternative to the [³H]-thymidine release assay, the [⁵¹Cr]-release assay, and the [³H]-thymidine DNA fragmentation assay.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 8 components.

Preparation Note

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.Storage conditions (working solution): Storage of coated plates: 1 week at 2 to 8 °C.The anti-BrdU-POD stock solution is stable at 2 to 8 °C for several months; for long- term storage it is recommended to store the solution in aliquots at -15 to -25 °C;The anti-BrdU-working solution should not be stored.For the anti-DNA-clone MCA-33 we do no have stability data. However it should be no problem to aliquot and store this non-conjugated antibody at -15 to -25 °C for longer storage.