Biochem/physiol Actions

Primary TargetOxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

General description

Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO–) is a strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. It has been implicated in a variety of patho-physiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock. This product decomposes or isomerizes at pH 8 or less; the half-life at pH 7.5 is ~1.5 s. ε^{302 nm} = 1670^M-1cm^-1 in 0.1 MNaOH.

Note: 1 set = 2 x 1 ml.

Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO^–) is a cell-permeable strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. Has been implicated in a variety of pathophysiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Marla, S.S., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14243.Sakuma, S., et al. 1997. Biochem. Biophys. Res. Commun. 230, 476.Lymar, S.V., et al. 1996. Biochemistry 35, 7855.Uppu, R.M., and Pryor, W.A. 1996. Methods Enzymol. 269, 322.Ischiropoulos, H., and al-Medhi, A.B. 1995. FEBS Lett. 364, 279.

Packaging

Packaged under inert gas

1 set in Alu drum

Physical form

Supplied in 4.7% NaOH at 160-200 mM.

Preparation Note

Peroxynitrite is soluble in aqueous buffers that have been pre-purged with an inert gas and pre-cooled to 4°C.

Reconstitution

Protect from heat, light, metal ions, and air. Peroxynitrite is unstable; thaw rapidly, dispense into aliquots, purge tubes with an inert gas, and freeze -70°C. Activity decreases ~2% /day at -20°C.

Warning

Toxicity: Oxidizer (K)