Biochem/physiol Actions

Primary TargetActivation of the Hedgehog signaling pathway

Product does not compete with ATP.

EC50 = 1 µM for inducing osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2

Cell permeable: yes

General description

A cell-permeable purine compound that induces osteoblast differentiation in C3H10T1/2 multipotent mesenchymal progenitor cells (EC₅₀ = 1 µM) and in MC3T3-E1 lineage-committed preosteoblasts. Also shown to induce transdifferentiation in pre-adipocytes and myoblasts. Its effects are reported to be synergistic with bone morphogenetic protein-4 (BMP-4). Reported to induce osteogenesis by activation of the Hedgehog signaling pathway.

A cell-permeable 2,6,9-trisubstituted purine compound that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM) and lineage-committed preosteoblasts MC3T3-E1. Its effect can be synergized with that of BMP-4 (bone morphogenetic protein-4) and has been shown to induce transdifferentiation even in preadipocytes and myoblasts. Reported to induce osteogenesis by activation of the Hedgehog signaling pathway. A 50 mM (5 mg/192 µl) solution of Purmorphamine (Cat. No. 540223) in DMSO is also available.Please note that the molecular weight for this compound is batch-specific due to variable water content.

A cell-permeable activator of Hedgehog signaling that induces osteoblast differentiation of multipotent mesenchymal progenitor cells C3H10T1/2 (EC₅₀ = 1 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Wu, X., et al. 2004. Chem. Biol.11, 1229.Wu, X., et al. 2002. J. Am. Chem. Soc.124, 14520.

Packaging

5 mg in Plastic ampoule

20 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Harmful (C)