PFM01

Code: SML1735-25MG D2-231

Biochem/physiol Actions

PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease a...


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€558.00 25MG
€686.34 inc. VAT

Biochem/physiol Actions

PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 µM), but not the exonuclease inhibitors Mirin (500 µM) and PFM39 (100 µM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed.

Packaging

5, 25 mg in glass bottle

assay≥98% (HPLC)
colorwhite to beige
formpowder
Quality Level100
SMILES stringOC1=CC=C(/C=C2SC(N(CC(C)C)C2=O)=S)C=C1
solubilityDMSO: 20 mg/mL, clear
storage temp.2-8°C
Cas Number1558598-41-6
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