Biochem/physiol Actions

Reversible: no

Target IC₅₀: 14 µM against mitotic kinesin Eg5

Product does not compete with ATP.

Primary TargetMitotic kinesin Eg5

Cell permeable: yes

General description

A potent, cell-permeable, non-tubulin-interacting mitosis inhibitor. Arrests mammalian cells in mitosis with monopolar spindles. In vitro, it specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein involved in the assembly and maintenance of the mitotic spindle (IC₅₀ = 14 µM).

A potent, cell-permeable, nontubulin-interacting mitosis inhibitor. Arrests mammalian cells in mitosis with monopolar spindles. In vitro, it specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein involved in the assembly and maintenance of the mitotic spindle (IC₅₀ = 14 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kapoor, T.M., et al. 2000. J. Cell Biol.150, 975.Mayer, T.U., et al. 1999. Science286, 971.

Packaging

1, 5 mg in Plastic ampoule

Packaged under inert gas

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)