Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Target IC₅₀: 5-7 µM 5-7 µM for activating phospholipase A2 and inhibits protein kinase C

General description

A 26-residue polypeptide from bee venom that binds calmodulin in a Ca²⁺-dependent manner. A stimulator of G_iα and G₁₁α activity that is reported to inhibit adenylate cyclase activity in synaptic membranes. Activates phospholipase A₂ and inhibits protein kinase C (IC₅₀ = 5-7 µM) by binding to the catalytic domain in a Mg²⁺-ATP sensitive manner. Has also been used for affinity purification of several Ca²⁺-binding proteins.

A 26-residue polypeptide from bee venom that binds calmodulin in a Ca²⁺-dependent manner, activates phospholipase A₂, and inhibits protein kinase C (IC₅₀ = 5-7 µM) by binding to the catalytic domain in a Mg²⁺-ATP sensitive manner. A stimulator of G_iα and G₁₁α activity that is reported to inhibit adenylate cyclase activity in synaptic membranes. Has also been used for affinity purification of several Ca²⁺-binding proteins. Activates G_iα-1 and G₁₁α activities and inhibits G_s activity.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Fukushima, N., et al. 1998. Peptides19, 811.Gravitt, K.R. 1994. Biochem. Pharmacol. 47, 425.Baudier, J., et al. 1987. Biochemistry26, 2886.Kincaid, R.L., and Coulson, C.C. 1985. Biochem. Biophys. Res. Commun.133, 256.

Packaging

250 µg in Glass bottle

Yes

Sequence

H-Gly-Ile-Gly-Ala-Val-Leu-Lys-Val-Leu-Thr-Thr-Gly-Leu-Pro-Ala-Leu-Ile-Ser-Trp-Ile-Lys-Arg-Lys-Arg-Gln-Gln-NH₂

Warning

Toxicity: Standard Handling (A)