Mastoparan

Biochem/physiol Actions

Reversible: no

Primary TargetNa⁺,K⁺-ATPase activity

Product does not compete with ATP.

Cell permeable: yes

General description

A cell-permeable synthetic peptide with a sequence identical to Vespula lewisii. Amphiphilic wasp venom tetradecapeptide capable of directly activating pertussis toxin-sensitive G-proteins by a mechanism analogous to that of G-protein-coupled receptors. Acts preferentially on G_i and G_o rather than G_s. Potent factor in mitochondrial permeability transition pore. Inhibits calmodulin and activates phospholipase A₂. Causes a transient Ca²⁺ release from the sarcoplasmic reticulum.

A cell-permeable synthetic peptide with sequence identical to Vespula lewisii. Amphiphilic wasp venom tetradecapeptide capable of directly activating pertussis toxin-sensitive G-proteins by a mechanism analogous to that of G-protein-coupled receptors. Acts preferentially on G_i and G_o rather than G_s. Potent facilitator of the mitochondrial permeability transition pore that is reported to induce apoptosis in cultured cerebellar granule neurons by causing a dramatic increase in intracellular Ca²⁺. Acts as a calmodulin antagonist and activates phospholipase A₂. Causes a transient Ca²⁺ release from the sarcoplasmic reticulum. Also reported to inhibit Na⁺,K⁺-ATPase activity (IC₅₀ = 7.5 µM).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hirata, Y., et al. 2000. Mol. Pharmacol. 57, 1235.Liz, S.Z., et al. 1997. Brain Res.771, 184.Klinker, J.F., et al. 1996. Biochem. Pharmacol. 51, 217.Langel, U., et al. 1996. Regul. Peptides 62, 47.Pfeiffer, D.R., et al. 1995. J. Biol. Chem. 270, 4923.Igarashi, M., et al. 1993. Science259, 77.Komatsu, M., et al. 1992. Endocrinology130, 221.Adolfo Garcia-Sainz, J., et al. 1991. Biochem. Biophys. Res. Commun.179, 852.Gil, J., et al. 1991. J. Cell Biol. 113, 943.

Packaging

1 mg in Plastic ampoule

Yes

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sequence

H-Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Lys-Ile-Leu-NH₂

Warning

Toxicity: Standard Handling (A)