Biochem/physiol Actions

Cell permeable: no

Primary TargetUsed as a ratiometric indicator of mitochondrial potential δψm in cells, tissues, and isolated mitochondria

Reversible: no

Product does not compete with ATP.

General description

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δψ_m in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of δψ_m induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δψ_m in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of δψ_m induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.Gravance, C.G., et al. 2000. Theriogenology53, 1691.Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.Ankacrona, M., et al. 1995. Neuron 15, 961.Reers, M., et al. 1995. Methods Enzymol. 260, 406.Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.

Packaging

Packaged under inert gas

5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.

Warning

Toxicity: Irritant (B)