General description

A cell-permeable indole compound that, when added (10 µM) to murine D3ES ESC (embryonic stem cell) cultures in the absence of serum and MEF (murine embryonic fibroblast) feeder cells, serves as a cheaper, chemical alternative to LIF (1,000 units/ml) for expanding ESCs in their undifferentiated state. Long-term murine ESCs maintained under ID-8 are capable of differentiating and EBs (Embryoid Bodies) formation upon ID-8 removal. Also claimed to maintain human ESCs in their pluripotent state in the absence of MEF.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Miyabayashi, T., et al. 2008. Biosci. Biotechnol. Biochem.72, 1242.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Warning

Toxicity: Standard Handling (A)