Human Internal Thoracic Artery Endothel

Application

coronary artery bypass grafting, myocardial revascularization, endothelial cell motility, transendothelial migration, gene expression, contractile responses, receptor stimulation, cell relaxation, supply of oxygenated blood to chest wall and breasts,

Cell Line Origin

Artery

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

HITAEC has been used to investigate oral pathogens travel through the body by infecting dendritic cells and inducing them to migrate through endothelial layers by causing upregulation of CXCR4 (Miles, 2014). To identify genes that make Human Internal Thoracic Arteries resistant to atherosclerosis, HITAEC and HCAEC (derived from the athero-prone human coronary arteries) were subjected to microarray analysis (Archaki, 2012). Twenty-nine genes were found to be expressed differently between HITAEC and HCAEC; one of them (TES) was shown to be downregulated in patients with coronary artery disease, resulting in increased monocyte adhesion, endothelial cell motility and transendothelial migration of monocytes.

Preparation Note

2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HITAEC Culture Protocol.