Application

Cell Detachment:

1. Thaw Accutase^® to room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.

General description

ESGRO Complete^™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

cOmplete is a trademark of Roche

Physical form

Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

1X Accutase^® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA 4Na and 3 mg/L Phenol Red.

Storage and Stability

Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase^® may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.