Biochem/physiol Actions

Cell permeable: no

Target IC₅₀: 5 nM Inhibiting the expression of inducible but not constitutive nitric oxide synthase in vascular endothelial cells

Reversible: no

Primary TargetPhosphorylation of rb protein

Product does not compete with ATP.

General description

Most active and highly stable glucocorticoid. Inhibits the expression of inducible but not constitutive nitric oxide synthase in vascular endothelial cells (IC₅₀ = 5 nM). Enhances active cation transport in aortic smooth muscle cells by stimulating the Na⁺-K⁺ pump. Has anti-inflammatory and antirheumatic properties. Induces apoptosis in human thymocytes. In general, 500-1000 nM of dexamethasone is sufficient to induce apoptosis following a 6 h incubation at 37°C.

Most active and highly stable glucocorticoid. Causes a reduction in cyclin A and Cdk2 activity, and inhibition of G₁/S transition in osteoblasts. Inhibits the phosphorylation of retinoblastoma (Rb) protein. Inhibits the expression of inducible but not constitutive nitric oxide synthase in vascular endothelial cells (IC₅₀ = 5 nM). Enhances active cation transport in aortic smooth muscle cells by stimulating the Na⁺ - K⁺ pump. Induces apoptosis in human thymocytes.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Reil, T.D., et al. 2000. J. Surg. Res.92, 108.Smith, E., et al. 2000. J.Biol. Chem. 275, 1992.Petit, P.X., et al. 1995. J. Cell Biol.130, 157.Stern, N., et al. 1994. Am. J. Hypertens. 7, 146.Donis, J.A., et al. 1993. Biotechniques15, 786.Ohoka, Y., et al. 1993. Biochem. Biophys. Res. Commun.197, 916.Sarin, A., et al. 1993. J. Exp. Med.178, 1693.Vandewalle, A., et al. 1993. J. Cell Physiol.156, 383.Nieto, M.A., et al. 1992. Clin. Exp. Immunol.88, 341.Walsh, D., and Avashia, J. 1992. Cleveland Clin. J. Med. 59, 505.Warters, R.L. 1992. Cancer Res.52, 883.Sperber, K., et al. 1991. J. Allergy Clin. Immunol.87, 490.Radomski, M.W., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 10043.Bettger, W.J., et al. 1981. Proc. Natl. Acad. Sci. USA78, 5588.

Packaging

100 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Warning

Toxicity: Carcinogenic / Teratogenic (D)