Biochem/physiol Actions

Primary Targettrasnslation of RNA

Potency: ≥970 µg/mg

General description

Synthetic bacteriostatic antibiotic that inhibits the translation of RNA by blocking the peptidyltransferase reaction on ribosomes.

Synthetic bacteriostatic antibiotic that inhibits the translation of RNA to protein by binding to the 50S ribosomal subunit, blocking the peptidyl transferase reaction. Chloramphenicol resistance is encoded by the resistance gene cat, whose product, chloramphenicol acetyltransferase, inactivates the antibiotic via acetylation. Typically used at 30-50 µg/ml for selection of chloramphenicol-resistant Escherichia coli.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1, (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.Lu, J., and Jiang, C. 1993. Biochem. Biophys. Res. Commun.196, 12.Saltarelli, M.J., et al. 1993. Virol.197, 35.Holt, J.T. 1992. Ann. N.Y. Acad. Sci.660, 88.Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, NY, p. 1.6, A.6.

Packaging

25 g in Plastic ampoule

100, 500 g in Glass bottle