Analysis Note

Appearance - clearness: clear - colour: colourlesspH-value (25 °C): 5.3 - 5.7Typical composition (per vial): Cefixim 0.025 mg; Potassium Tellurite 1.25 mg.Growth promotion test in accordance with ISO 11133. Supplement tested with base Art. 1.09207.Inoculum on reference medium - Escherichia coli ATCC 35150 (O157): 10 - 100 - Escherichia coli O157:H7 ATCC 43894: 10 - 100 - Escherichia coli O157:H7 ATCC 43895: 10 - 100Inoculum - Escherichia coli ATCC 11775 (WDCM 00090): >10⁴ - Escherichia coli ATCC 25922 (WDCM 00013): >10⁴ - Staphylococcus aureus ATCC 6538 (WDCM 00032): >10⁴ - Staphylococcus aureus ATCC 25923 (WDCM 00034): >10⁴Recovery on test medium - Escherichia coli ATCC 35150 (O157): ≥ 50% - Escherichia coli O157:H7 ATCC 43894: ≥ 50% - Escherichia coli O157:H7 ATCC 43895: ≥ 50%Growth - Escherichia coli ATCC 11775 (WDCM 00090): partly inhibition - Escherichia coli ATCC 25922 (WDCM 00013): partly inhibition - Staphylococcus aureus ATCC 6538 (WDCM 00032): total inhibition - Staphylococcus aureus ATCC 25923 (WDCM 00034): total inhibitionColony colour - Escherichia coli ATCC 35150 (O157): colourless - Escherichia coli O157:H7 ATCC 43894: colourless - Escherichia coli O157:H7 ATCC 43895: colourless - Escherichia coli ATCC 11775 (WDCM 00090): pink - Escherichia coli ATCC 25922 (WDCM 00013): pinkIncubation: 24 hrs.; 37 °C; aerobic. Reference: Tryptic-soy-agar. Inhibited strains tested with three-loop-streak.

Application

CT-Supplement is an additive for the preparation of CT-SMAC Agar for the isolation and differentiation of enterohemorrhagic (EHEC) E. coli 0157:H7-strains from foodstuffs and stool.

General description

CT-Supplement contains cefixime and potassium tellurite. It is used in combination with Sorbitol MacConkey Agar (SMCA) to enhance the isolation of Escherichia coli O157.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 1092020017

Packaging

10 vial in Fibre case